Wisent cDNA Synthesis Kit uses the latest technology in reverse transcription buffer chemistry to enhance cDNA synthesis speed and yield with accurate transcript representation. The reverse transcriptase, buffer system and combination of random hexamers with anchored oligo(dT) allow for unbiased, efficient, sensitive cDNA synthesis.
High quality cDNA synthesis for downstream qPCR analysis is essential for successful expression studies. cDNA synthesis is affected by the reverse transcriptase, buffer systems, enhancers and priming strategy. The Wisent cDNA synthesis mix removes the need for user optimization of these critical factors.
Wisent RTase is both thermostable and extremely active. The enzyme is blended with RNase inhibitor.
The RTase is not inhibited by ribosomal and transfer RNAs, total RNA is an ideal substrate. The 5x cDNA synthesis mix can be used with up to 4.0μg total RNA. The relative concentrations of random hexamers and anchored oligo(dT) have been optimized for the generation of cDNA for use in real-time PCR experiments.