LB Broth (Miller) solution, 1X , STERILE
LB Broth (Miller) is a microbial growth medium used for the cultivation of E. coli. It is mainly used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. The NaCl component add essential electrolytes for transport and osmotic balance.
| Microorganisms | EXPECTED RESULTS | GROWTH* |
| Escherichia coli ATCC 23724 | Good to excellent | Excellent |
| Escherichia coli ATCC 33694 | Good to excellent | Excellent |
| Escherichia coli ATCC 33849 | Fair to excellent | Excellent |
| Escherichia coli ATCC 39403 | Good to excellent | Excellent |
| Escherichia coli ATCC 47014 | Good to excellent | Excellent |
| Escherichia coli ATCC 53868 | Good to excellent | Very Good |
The following results were obtained from type cultures after incubation at a temperature of 35°C and observed after 18-24 hours.
FORMULA
| Sodium Chloride | 10g/L |
| Tryptone | 10g/L |
| Yeast Extract | 5g/L |
| USAGE: | 25g/L |
Contents & Storage
Storage conditions: 15 to 30° C.
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture